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Tier D Cognitive

PE-22-28

Spadin Analogue — Antidepressant Peptide

Spadin-derived TREK-1 channel blocker — fast-acting antidepressant effects in animal models, unique non-monoaminergic mechanism.

Tier D — Preclinical Only: Animal or in-vitro data only. How our tiers work →

Research facts

Half-lifeUnknown
RouteSubQ / Nasal (experimental)
Typical doseNo established human dose
FrequencyUnknown
SolventBacteriostatic water
pHN/A
StabilityUnknown

Mechanism of action

PE-22-28 is a synthetic analogue of spadin, a peptide derived from the NTSR3/sortilin protein. It acts as a selective blocker of the TREK-1 potassium channel — a two-pore domain potassium channel that is overactive in depression and whose activity is downregulated by conventional antidepressants. TREK-1 blockade promotes neuroplasticity and has demonstrated rapid antidepressant effects in multiple animal models (comparable to classical antidepressants but with faster onset). PE-22-28 is a potency-optimised, metabolically stabilised analogue of spadin with enhanced CNS penetration. It represents a genuinely novel antidepressant mechanism.

Documented effects (research-model)

  • Rapid antidepressant effects in animal models
  • Novel TREK-1 potassium channel mechanism (non-monoaminergic)
  • Potential for faster onset than conventional antidepressants
  • Preclinical evidence in multiple depression models

Research protocols

ProtocolDoseFrequencyCycleVial
Experimental Only No human dose established Unknown Unknown N/A

Research context only. Not medical advice. Consult a qualified healthcare professional before any protocol decision.

Synergies

  • Selank MODERATE
    PE-22-28 (antidepressant via TREK-1) + Selank (anxiolytic via GABA/serotonin). Potential combination for comorbid depression-anxiety.

Myths & misconceptions

Myth PE-22-28 is a validated antidepressant.
Reality All evidence is in animal models. No human clinical trials have been completed or published. It is a research compound only.

Evidence gaps

  • No human clinical data.
  • TREK-1 has roles beyond neurological function — systemic effects unknown.
  • Optimal delivery route for CNS access in humans not characterised.

Safety notes

No human safety data. Very early preclinical stage. Research context only.

Biomarkers to monitor

CortisolFull Blood Count

Primary-source citations

  • PMID 27748714 — PE-22-28, a spadin analogue with improved antidepressant activity (Br J Pharmacol. 2017)

Frequently asked

What is PE-22-28?

PE-22-28 (Spadin Analogue — Antidepressant Peptide) is a research compound classified in our library as Tier D — Animal or in-vitro data only.. Spadin-derived TREK-1 channel blocker — fast-acting antidepressant effects in animal models, unique non-monoaminergic mechanism.

What is the evidence tier for PE-22-28?

We classify PE-22-28 as Tier D: Animal or in-vitro data only. See our full peptide evidence tiers explainer for how we assign S/A/B/C/D.

What is the research dose of PE-22-28?

For PE-22-28, typical research dose is No established human dose, route is SubQ / Nasal (experimental), half-life is Unknown. Protocols vary by research goal — see the protocols section on this page for standard and advanced dosing schedules. Research use only, not medical advice.

Is PE-22-28 safe?

No human safety data. Very early preclinical stage. Research context only — no compound on this site is approved for human therapeutic use unless explicitly noted.

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